Proprietary Protein Purification Protocol for Food, Biotech & Pharma

Purification accounts for 50% to 80% of the total production cost of a therapeutic protein. The Aybar Protocol (AP) is a new method for purifying macromolecules with ionic functional groups by high performance liquid chromatography, and it attacks that cost directly: more product recovered, in fewer steps, using less buffer, on equipment you already own.

What the Aybar Protocol Does

High-Yield Protein Isolation

The protocol was developed and tested on the enzyme HGPRT’ase and has since been applied to several other enzymes. In the reference comparison against hydroxylapatite-matrix chromatography, total activity recovered after the purification step was an order of magnitude higher, in a single step rather than five.

GMP-Compliant, Pharma-Ready Purification

Because AP is a protocol rather than a device, it runs inside existing validated environments. It requires a fast protein liquid chromatography instrument, or any system with programmable injection, programmable buffer gradient, a pumping system, and preparatory columns packed with a suitable ion exchange resin. No new machinery, no new facility, no capital line item.

Applications

Functional Food Ingredients

Collagen, soy, and pea protein streams where yield and clean-label processing determine margin.

Nutraceutical Protein Extraction

Enzymes and immune peptides that are currently uneconomic to purify at scale.

Biotech & Therapeutic Proteins

Interferons, immunoglobulins, and monoclonal antibodies, where purification dominates cost of goods.

Plant-Based & Alt-Protein Processing

Emerging protein sources whose commercial viability depends on extraction economics rather than on raw material supply.

Why It Outperforms Standard Chromatography

Older methods such as hydroxylapatite chromatography and molecular sieving carry a specific set of drawbacks: high buffer consumption, low flow rates, loss of protein activity during the process, heavy dilution of the extract, difficult or impossible column regeneration, and a low purification fold that leaves debris polypeptides behind. That last one compounds, because it shortens the life of the expensive affinity column in the final step.

Works With Existing FPLC/HPLC Infrastructure

Adoption cost is the protocol itself, not a retooling programme.

The Process Can Be Automated Semi-Continuously

AP is compatible with programmable, semi-continuous operation, which is what turns a laboratory advantage into a production one.

Market Opportunity

Protein purification sits inside a global market for food, nutraceutical, and therapeutic proteins measured in the hundreds of billions of dollars. Our addressable slice is not the protein market itself but the purification cost inside it, which is the half to four-fifths of production cost that AP is designed to compress.

Licensing & Purification-as-a-Service

We are looking for a partner to use or exploit this technique, and we are willing to be paid out of the savings it produces. As evidence of confidence in the method, we will implement the procedure on one of your protein products at no cost to you, provided you supply the starting material and the facility.